rfaE基因在副猪嗜血杆菌LOS刺激猪肺泡巨噬细胞炎性因子mRNA转录中的作用

doi:10.11843/j.issn.0366-6964.2015.07.021

Abstract

Abstract:

The aim of this study was to study the role of rfaE gene of Haemophilus parasuis lipooligosaccharide (LOS) which induced pro-inflammatory cytokine (interleukin (IL)-1α，IL-1β，IL-6，IL-8 and tumor necrosis factor (TNF)-α) mRNA transcription in porcine alveolar macrophages (PAMs).The LOS of H.parasuis SC096 strain，rfaE mutant and its complementation was extracted.The PAMs were stimulated with LOS (5 μg and 10 μg) from H.parasuis SC096，ΔrfaE mutant (ΔrfaE) and its complementation (cΔrfaE) and LPS from Escherichia coli for 2，4，6，12 and 24 h.The RNA was extracted and reversed into cDNA.The mRNA transcription of IL-1α，IL-1β，IL-6，IL-8 and TNF-α were then detected by Real-time PCR.The result showed that the mRNA transcription of IL-1α in PAMs induced by 5 μg LOS from H.parasuis for 2，4，6，12 and 24 h were up-regulated，and significantly higher than the mRNA transcription of IL-1α in PAMs induced by 5 μg LOS from ΔrfaE (P<0.05)， the mRNA transcription of IL-1β，IL-6，IL-8 in PAMs induced by 5 μg LOS from H.parasuis for 12 and 24 h were up-regulated，and remarkably higher than the mRNA transcription of IL-1α in PAMs induced by 5 μg LOS from ΔrfaE (P<0.05)，the mRNA transcription of TNF-α in PAMs induced by 5 μg LOS from H.parasuis for 24 h was up-regulated，and notably higher than the mRNA transcription of IL-1α in PAMs induced by 5 μg LOS from ΔrfaE (P<0.05)；the 10 μg LOS from H.parasuis up-regulated mRNA transcription of IL-1α，IL-1β，IL-6，IL-8 and TNF-α in PAMs，and were significantly higher than that of 10 μg LOS from ΔrfaE mutant (P<0.05).At the same time，the cytokine mRNA transcription levels in PAMs induced by LOS from cΔrfaE were restored to the level in PAMs induced by LOS from H.parasuis.The date firstly confirmed that the rfaE gene were involved in the pro-inflammatory cytokine mRNA transcription in PAMs induced by H.parasuis LOS，suggesting that the rfaE gene play an important role in the pathogenesis of H.parasuis LOS.

CLC Number:

S852.613

ZENG Ze，YUE Hua，FENG Xiao-hui，HE Huan，ZHANG Bin. The Effect of rfaE Gene in Haemophilus parasuis LOS Induces Pro-inflammatory Cytokine mRNA Transcription in Porcine Alveolar Macrophages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1232-1237.

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The Effect of rfaE Gene in Haemophilus parasuis LOS Induces Pro-inflammatory Cytokine mRNA Transcription in Porcine Alveolar Macrophages

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